Lipid Monolayer on Cell Surface Protein Templates Functional Extracellular Lipid Assembly

Anupma Dwivedi, Anisha Mazumder, Petra Pullmannová, Anna Paraskevopoulou, Lukáš Opálka, Andrej Kováčik, Miloslav Macháček, Pavla Jančálková, Petra Svačinová, Herwig Peterlik, Jaroslav Maixner, Kateřina Vávrová

When the ancestors of men moved from aquatic habitats to the drylands, their evolutionary strategy to restrict water loss is to seal the skin surface with lipids. It is unknown how these rigid ceramide-dominated lipids with densely packed chains squeeze through narrow extracellular spaces and how they assemble into their complex multilamellar architecture. Here it is shown that the human corneocyte lipid envelope, a monolayer of ultralong covalently bound lipids on the cell surface protein, templates the functional barrier assembly by partly fluidizing and rearranging the free extracellular lipids in its vicinity during the sculpting of a functional skin lipid barrier. The lipid envelope also maintains the fluidity of the extracellular lipids during mechanical stress. This local lipid fluidization does not compromise the permeability barrier. The results provide new testable hypotheses about epidermal homeostasis and the pathophysiology underlying diseases with impaired lipid binding to corneocytes, such as congenital ichthyosis. In a broader sense, this lipoprotein-mediated fluidization of rigid (sphingo)lipid patches may also be relevant to lipid rafts and cellular signaling events and inspire new functional materials.

Dynamics of Condensed Systems
External organisation(s)
Charles University Prague, University of Chemistry and Technology, Prague
No. of pages
Publication date
Peer reviewed
Austrian Fields of Science 2012
103015 Condensed matter
ASJC Scopus subject areas
Biotechnology, Chemistry(all), Biomaterials, Materials Science(all), Engineering (miscellaneous)
Sustainable Development Goals
SDG 3 - Good Health and Well-being
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